In the treatment of infected nonunions, the goals are to eliminate the infection while stimulating bony union. Platelet-rich gel (PRG) enrich with growth factors represents novel autologous treatment option that could be applicable in the treatment of disturbances of the healing processes. Despite tibial infected nonunion with fistula after percutaneous autologous PRG grafting into gap bone and soft tissue defect was healed. This case is the first report of the application of PRG in an infected nonunion. In our opinion PRG is inductive biomaterial with antimicrobial activity, which might be used in the treatment of local bone and wound infection.