Bioprospecting of marine resources for biopolymer production

11^th International Conference on Arthroplasty

September 24-25, 2018 London, UK

Sourish Bhattacharya

CSIR-Central Salt and Marine Chemicals Research Institute, India

ScientificTracks Abstracts: J Arthroplasty

Abstract :

West coast of India can be considered as important area for collecting diverse marine microorganisms with biopolymer producing capacity. In this attempt, 200 marine isolates from west coast of India was obtained, out of which one strain CSMCRI’s Bacillus licheniformis PL26 was found to be potential for producing ε-polylysine and polyhydroxyalkanoate simultaneously in same fermentation medium. The PHA produced by Bacillus licheniformis was found to be Poly-3-Hydroxybutyrate (P3HB). Further, in order to improve the ε-polylysine production, the carbon source was replaced with glucose which yielded 1.2 g L-1 ε-polylysine as oxygen transfer rate is very low in the medium containing crude glycerol. Further, an advanced modeling and optimization technique was applied to optimize medium parameters for enhanced ε-polylysine production by marine bacterium Bacillus licheniformis. The critical nutrients including glucose, yeast extract, magnesium sulfate and ferrous sulfate were incorporated in Artificial Neural Networks (ANN) as input variables and ε-polylysine as the output variable. The ANN topology of 4-10-1 was found to be optimum upon training the model with feed-forward back propagation algorithm and on application of the developed model to particle swarm optimization resulted in 3.56±0.16 g L-1 of ε-polylysine under the following optimal conditions: Glucose 34 g L-1; Yeast extract 2.3 g L-1; Magnesium sulfate 0.44 g L-1 and Ferrous sulfate 0.08 g L-1. Thus, this optimization technique could significantly improve ε-polylysine by 196.7% as compared to un-optimized medium.

Biography :

Sourish Bhattacharya is a Junior Scientist at Process Design and Engineering Cell Discipline in CSIR-Central Salt and Marine Chemicals Research Institute, India.

E-mail: sourishb@csmcri.res.in sourish.bhattacharya@gmail.com